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Figure
1: PAO1 mini-TN5 lux mutant library.
Black outer histograms are transposon insertion
points. Outer red histograms represent forward
strand transcriptional fusions while inner
inner red histograms represent reverse strand
transcriptional fusions. Green histograms
are forward strand (outer) and reverse strand
(inner) non-transcriptional fusions of transposons
with ORFs. Inner blue historgram track -
intergenic insertions.
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Table
1: Pseudomonas aeruginosa PAO1
Tn5 lux Transposon Mutant
Library Statistics
Feature |
N |
Library
Size |
9984 |
Samples
Processed Through Inverse PCR |
4608 |
DNA
Sequencing Reactions |
3325 |
Mapped
Insertion Sites |
2519 |
Insertions
Within a Gene |
2275 |
Intergenic
Insertions |
244 |
Transcriptional
luxCDABE Fusions |
1253 |
Unique
Insertion Sites |
1458 |
ORFs
Inactivated |
1284 |
Unique
Intergenic Insertions |
174 |
Predicted
PAO1 ORFs |
5570 |