Functional Pathogenomics of Mucosal Immunity    R.E.W Hancock Laboratory    The Brinkman Laboratory

Pseudomonas aeruginosa PAO1mini-Tn5 lux transposon mutant library

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Figure 1: PAO1 mini-TN5 lux mutant library. Black outer histograms are transposon insertion points. Outer red histograms represent forward strand transcriptional fusions while inner inner red histograms represent reverse strand transcriptional fusions. Green histograms are forward strand (outer) and reverse strand (inner) non-transcriptional fusions of transposons with ORFs. Inner blue historgram track - intergenic insertions.

Table 1: Pseudomonas aeruginosa PAO1 Tn5 lux Transposon Mutant Library Statistics

Feature
N
Library Size
9984
Samples Processed Through Inverse PCR
4608
DNA Sequencing Reactions
3325
Mapped Insertion Sites
2519
Insertions Within a Gene
2275
Intergenic Insertions
244
Transcriptional luxCDABE Fusions
1253
Unique Insertion Sites
1458
ORFs Inactivated
1284
Unique Intergenic Insertions
174
Predicted PAO1 ORFs
5570