Functional Pathogenomics of Mucosal Immunity R.E.W Hancock Laboratory The Brinkman Laboratory

Pseudomonas aeruginosa PAO1 mini-Tn5 lux transposon mutant library

2-21 22-41 42-61 62-81 82-101 102-121

Record #	Mutant ID	Region type	Region name	PA number	Product name	Function class	Gene Strand	Insertion Point	Transposon Orientation
2	PAO1_lux_67_E7	intergenic	PA0006-PA0007					8574	N/A

Alignment: PAO1_lux_67_E7 against Pseudomonas aeruginosa PAO1 genome
Pool ID	Algorithm	Query Start	Query End	Hit Start	Hit End	Expect	Score	Percent identity	HSP length	Query Strand Orientation	Hit Strand Orientation
Pool_10_F7	BLASTN	77	424	8574	8227	0e+00	284	95.40	348	1	-1

Record #	Mutant ID	Region type	Region name	PA number	Product name	Function class	Gene Strand	Insertion Point	Transposon Orientation
3	PAO1_lux_82_H1	intergenic	PA0006-PA0007					8592	N/A

Alignment: PAO1_lux_82_H1 against Pseudomonas aeruginosa PAO1 genome
Pool ID	Algorithm	Query Start	Query End	Hit Start	Hit End	Expect	Score	Percent identity	HSP length	Query Strand Orientation	Hit Strand Orientation
Pool_31_E7	BLASTN	57	495	8592	8156	0e+00	327	94.08	439	1	-1

Record #	Mutant ID	Region type	Region name	PA number	Product name	Function class	Gene Strand	Insertion Point	Transposon Orientation
4	PAO1_lux_20_D11	intergenic	PA0006-PA0007					8599	N/A

Alignment: PAO1_lux_20_D11 against Pseudomonas aeruginosa PAO1 genome
Pool ID	Algorithm	Query Start	Query End	Hit Start	Hit End	Expect	Score	Percent identity	HSP length	Query Strand Orientation	Hit Strand Orientation
Pool_4_G2	BLASTN	54	639	8599	8015	0e+00	546	98.46	586	1	-1

Record #	Mutant ID	Region type	Region name	PA number	Product name	Function class	Gene Strand	Insertion Point	Transposon Orientation
5	PAO1_lux_74_G2	gene	trkA	PA0016	potassium uptake protein TrkA	Transport of small molecules	-	18467	Non-transcriptional fusion

Alignment: PAO1_lux_74_G2 against Pseudomonas aeruginosa PAO1 genome
Pool ID	Algorithm	Query Start	Query End	Hit Start	Hit End	Expect	Score	Percent identity	HSP length	Query Strand Orientation	Hit Strand Orientation
Pool_23_C5	BLASTN	64	154	18467	18557	2e-45	91	100.00	91	1	1

Record #	Mutant ID	Region type	Region name	PA number	Product name	Function class	Gene Strand	Insertion Point	Transposon Orientation
6	PAO1_lux_20_B5	gene	PA0017	PA0017	conserved hypothetical protein	Hypothetical, unclassified, unknown	-	19959	Transcriptional fusion

Alignment: PAO1_lux_20_B5 against Pseudomonas aeruginosa PAO1 genome
Pool ID	Algorithm	Query Start	Query End	Hit Start	Hit End	Expect	Score	Percent identity	HSP length	Query Strand Orientation	Hit Strand Orientation
Pool_3_B11	BLASTN	46	124	19959	20037	3e-38	79	100.00	79	1	1

Record #	Mutant ID	Region type	Region name	PA number	Product name	Function class	Gene Strand	Insertion Point	Transposon Orientation
7	PAO1_lux_1_F4	gene	PA0021	PA0021	conserved hypothetical protein	Hypothetical, unclassified, unknown	+	23396	Non-transcriptional fusion

Alignment: PAO1_lux_1_F4 against Pseudomonas aeruginosa PAO1 genome
Pool ID	Algorithm	Query Start	Query End	Hit Start	Hit End	Expect	Score	Percent identity	HSP length	Query Strand Orientation	Hit Strand Orientation
Pool_32_A10	BLASTN	14	44	23396	23426	1e-09	31	100.00	31	1	1

Record #	Mutant ID	Region type	Region name	PA number	Product name	Function class	Gene Strand	Insertion Point	Transposon Orientation
8	PAO1_lux_17_G12	gene	qor	PA0023	quinone oxidoreductase	Energy metabolism	-	25025	Non-transcriptional fusion

Alignment: PAO1_lux_17_G12 against Pseudomonas aeruginosa PAO1 genome
Pool ID	Algorithm	Query Start	Query End	Hit Start	Hit End	Expect	Score	Percent identity	HSP length	Query Strand Orientation	Hit Strand Orientation
Pool_12_G5	BLASTN	361	604	25025	25269	0e+00	237	99.59	245	1	1

Record #	Mutant ID	Region type	Region name	PA number	Product name	Function class	Gene Strand	Insertion Point	Transposon Orientation
9	PAO1_lux_1_H6	gene	hemF	PA0024	"coproporphyrinogen III oxidase, aerobic"	Biosynthesis of cofactors, prosthetic groups and carriers	+	26647	Transcriptional fusion

Alignment: PAO1_lux_1_H6 against Pseudomonas aeruginosa PAO1 genome
Pool ID	Algorithm	Query Start	Query End	Hit Start	Hit End	Expect	Score	Percent identity	HSP length	Query Strand Orientation	Hit Strand Orientation
Pool_32_B2	BLASTN	16	706	26647	25959	0e+00	454	91.76	692	1	-1

Record #	Mutant ID	Region type	Region name	PA number	Product name	Function class	Gene Strand	Insertion Point	Transposon Orientation
10	PAO1_lux_16_B8	gene	PA0033	PA0033	hypothetical protein	Hypothetical, unclassified, unknown	-	35955	Non-transcriptional fusion

Alignment: PAO1_lux_16_B8 against Pseudomonas aeruginosa PAO1 genome
Pool ID	Algorithm	Query Start	Query End	Hit Start	Hit End	Expect	Score	Percent identity	HSP length	Query Strand Orientation	Hit Strand Orientation
Pool_17_E1	BLASTN	65	727	35955	36617	0e+00	663	100.00	663	1	1

Record #	Mutant ID	Region type	Region name	PA number	Product name	Function class	Gene Strand	Insertion Point	Transposon Orientation
11	PAO1_lux_73_F12	gene	PA0034	PA0034	probable two-component response regulator	Two-component regulatory systems	-	36317	Non-transcriptional fusion

Alignment: PAO1_lux_73_F12 against Pseudomonas aeruginosa PAO1 genome
Pool ID	Algorithm	Query Start	Query End	Hit Start	Hit End	Expect	Score	Percent identity	HSP length	Query Strand Orientation	Hit Strand Orientation
Pool_23_C4	BLASTN	64	364	36317	36617	0e+00	301	100.00	301	1	1

Record #	Mutant ID	Region type	Region name	PA number	Product name	Function class	Gene Strand	Insertion Point	Transposon Orientation
12	PAO1_lux_1_B4	gene	PA0034	PA0034	probable two-component response regulator	Two-component regulatory systems	-	36502	Transcriptional fusion

Alignment: PAO1_lux_1_B4 against Pseudomonas aeruginosa PAO1 genome
Pool ID	Algorithm	Query Start	Query End	Hit Start	Hit End	Expect	Score	Percent identity	HSP length	Query Strand Orientation	Hit Strand Orientation
Pool_32_A6	BLASTN	9	314	36502	36807	0e+00	258	95.75	306	1	1

Record #	Mutant ID	Region type	Region name	PA number	Product name	Function class	Gene Strand	Insertion Point	Transposon Orientation
13	PAO1_lux_83_D1	gene	PA0034	PA0034	probable two-component response regulator	Two-component regulatory systems	-	36693	Transcriptional fusion

Alignment: PAO1_lux_83_D1 against Pseudomonas aeruginosa PAO1 genome
Pool ID	Algorithm	Query Start	Query End	Hit Start	Hit End	Expect	Score	Percent identity	HSP length	Query Strand Orientation	Hit Strand Orientation
Pool_26_E3	BLASTN	65	147	36693	36611	9e-41	83	100.00	83	1	-1

Record #	Mutant ID	Region type	Region name	PA number	Product name	Function class	Gene Strand	Insertion Point	Transposon Orientation
14	PAO1_lux_3_H11	gene	PA0034	PA0034	probable two-component response regulator	Two-component regulatory systems	-	36730	Transcriptional fusion

Alignment: PAO1_lux_3_H11 against Pseudomonas aeruginosa PAO1 genome
Pool ID	Algorithm	Query Start	Query End	Hit Start	Hit End	Expect	Score	Percent identity	HSP length	Query Strand Orientation	Hit Strand Orientation
Pool_32_G6	BLASTN	237	309	36730	36802	5e-06	25	83.56	73	1	1

Record #	Mutant ID	Region type	Region name	PA number	Product name	Function class	Gene Strand	Insertion Point	Transposon Orientation
15	PAO1_lux_131_A2	gene	PA0039	PA0039	hypothetical protein	Hypothetical, unclassified, unknown	+	40803	Transcriptional fusion

Alignment: PAO1_lux_131_A2 against Pseudomonas aeruginosa PAO1 genome
Pool ID	Algorithm	Query Start	Query End	Hit Start	Hit End	Expect	Score	Percent identity	HSP length	Query Strand Orientation	Hit Strand Orientation
Pool_35_E11	BLASTN	17	498	40803	40326	0e+00	431	98.13	482	1	-1

Record #	Mutant ID	Region type	Region name	PA number	Product name	Function class	Gene Strand	Insertion Point	Transposon Orientation
16	PAO1_lux_24_D5	gene	PA0041	PA0041	probable hemagglutinin	Secreted Factors (toxins, enzymes, alginate)	+	51281	Non-transcriptional fusion

Alignment: PAO1_lux_24_D5 against Pseudomonas aeruginosa PAO1 genome
Pool ID	Algorithm	Query Start	Query End	Hit Start	Hit End	Expect	Score	Percent identity	HSP length	Query Strand Orientation	Hit Strand Orientation
Pool_5_A8	BLASTN	44	883	51281	52121	0e+00	786	98.69	842	1	1

Record #	Mutant ID	Region type	Region name	PA number	Product name	Function class	Gene Strand	Insertion Point	Transposon Orientation
17	PAO1_lux_24_D6	gene	PA0041	PA0041	probable hemagglutinin	Secreted Factors (toxins, enzymes, alginate)	+	51290	Non-transcriptional fusion

Alignment: PAO1_lux_24_D6 against Pseudomonas aeruginosa PAO1 genome
Pool ID	Algorithm	Query Start	Query End	Hit Start	Hit End	Expect	Score	Percent identity	HSP length	Query Strand Orientation	Hit Strand Orientation
Pool_5_G8	BLASTN	51	798	51290	52037	0e+00	705	98.80	749	1	1

Record #	Mutant ID	Region type	Region name	PA number	Product name	Function class	Gene Strand	Insertion Point	Transposon Orientation
18	PAO1_lux_76_E4	gene	PA0041	PA0041	probable hemagglutinin	Secreted Factors (toxins, enzymes, alginate)	+	52112	Transcriptional fusion

Alignment: PAO1_lux_76_E4 against Pseudomonas aeruginosa PAO1 genome
Pool ID	Algorithm	Query Start	Query End	Hit Start	Hit End	Expect	Score	Percent identity	HSP length	Query Strand Orientation	Hit Strand Orientation
Pool_24_F6	BLASTN	63	267	52112	52316	0e+00	205	100.00	205	1	1

Record #	Mutant ID	Region type	Region name	PA number	Product name	Function class	Gene Strand	Insertion Point	Transposon Orientation
19	PAO1_lux_52_G10	gene	PA0041	PA0041	probable hemagglutinin	Secreted Factors (toxins, enzymes, alginate)	+	52231	Transcriptional fusion

Alignment: PAO1_lux_52_G10 against Pseudomonas aeruginosa PAO1 genome
Pool ID	Algorithm	Query Start	Query End	Hit Start	Hit End	Expect	Score	Percent identity	HSP length	Query Strand Orientation	Hit Strand Orientation
Pool_22_A1	BLASTN	182	265	52231	52314	3e-41	84	100.00	84	1	1

Record #	Mutant ID	Region type	Region name	PA number	Product name	Function class	Gene Strand	Insertion Point	Transposon Orientation
20	PAO1_lux_51_C7	intergenic	PA0041-PA0042					54306	N/A

Alignment: PAO1_lux_51_C7 against Pseudomonas aeruginosa PAO1 genome
Pool ID	Algorithm	Query Start	Query End	Hit Start	Hit End	Expect	Score	Percent identity	HSP length	Query Strand Orientation	Hit Strand Orientation
Pool_29_B4	BLASTN	85	364	54306	54030	1e-81	152	89.64	280	1	-1

Record #	Mutant ID	Region type	Region name	PA number	Product name	Function class	Gene Strand	Insertion Point	Transposon Orientation
21	PAO1_lux_76_D3	intergenic	PA0041-PA0042					55352	N/A

Alignment: PAO1_lux_76_D3 against Pseudomonas aeruginosa PAO1 genome
Pool ID	Algorithm	Query Start	Query End	Hit Start	Hit End	Expect	Score	Percent identity	HSP length	Query Strand Orientation	Hit Strand Orientation
Pool_24_H5	BLASTN	168	494	55352	55026	0e+00	279	96.33	327	1	-1

2-21 22-41 42-61 62-81 82-101 102-121